Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time-of-flight mass spectrometry.

Capillary isoelectric focusing (cIEF) was online coupled to a Q-TOF MS by a flow-through microvial interface for the analysis of therapeutic mAb.

Intact molecular weights obtained from the mass spectrum deconvolution of separated charge variants provided information on the structural heterogeneity of therapeutic mAbs. A sandwich cIEF-MS configuration composed of anolyte, sample, and catholyte segments sequentially injected into a neutrally coated capillary was used for the charge heterogeneity separation of four mAbs.

Acetic acid and ammonium hydroxide were used in places of the non-volatile acids and bases commonly used for IEF but are incompatible with online MS detection. Glycerol was added as the anti-convective reagent.

BrdU
MBS696381-5x1g 5x1g
EUR 805

Anti-BrdU/5-BrdU Antibody
MBS1569321-01mg 0.1mg
EUR 375

Anti-BrdU/5-BrdU Antibody
MBS1569321-5x01mg 5x0.1mg
EUR 1390

5-BrdU
B3589-1000 1 g
EUR 64
Description: Synthetic thymidine analog

5-BrdU
B3589-5.1 10 mM (in 1mL DMSO)
EUR 44
Description: Synthetic thymidine analog

5-BrdU
B3589-500 500 mg
EUR 40
Description: Synthetic thymidine analog

5-BrdU
HY-15910 5g
EUR 596.4

5-BrdU
T6794-10mg 10mg Ask for price
Description: 5-BrdU

5-BrdU
T6794-1g 1g Ask for price
Description: 5-BrdU

5-BrdU
T6794-1mg 1mg Ask for price
Description: 5-BrdU

5-BrdU
T6794-50mg 50mg Ask for price
Description: 5-BrdU

5-BrdU
T6794-5mg 5mg Ask for price
Description: 5-BrdU

5-BrdU
MBS385202-1g 1g
EUR 170

5-BrdU
MBS385202-500mg 500mg
EUR 140

5-BrdU
MBS385202-5x1g 5x1g
EUR 755

BrdU (Bu20a)
E8RT1081 100ul
EUR 275
Description: Available in various conjugation types.

BrdU (Bu20a)
MBS8534961-01mL 0.1mL
EUR 345

BrdU (Bu20a)
MBS8534961-01mLAF405L 0.1mL(AF405L)
EUR 565

BrdU (Bu20a)
MBS8534961-01mLAF405S 0.1mL(AF405S)
EUR 565

BrdU (Bu20a)
MBS8534961-01mLAF610 0.1mL(AF610)
EUR 565

BrdU (Bu20a)
MBS8534961-01mLAF635 0.1mL(AF635)
EUR 565

BrdU Slides
MBS396666-1Set 1Set
EUR 225

BrdU Slides
MBS396666-5x1Set 5x1Set
EUR 860

BrdU (Bromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU)
MBS6506850-01mg 0.1mg
EUR 970

BrdU (Bromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU)
MBS6506850-5x01mg 5x0.1mg
EUR 4210

BRDU antibody
10R-B121a 100 ug
EUR 506
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-2321 1 mL
EUR 600
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-2356 1.5 mL
EUR 588
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-6763 1 ml
EUR 441
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-7945 100 ug
EUR 457
Description: Mouse monoclonal BRDU antibody

BRDU antibody
20-BS17 250 ug
EUR 568
Description: Sheep polyclonal BRDU antibody

BRDU antibody
20R-BS001 250 ug
EUR 962
Description: Sheep polyclonal BRDU antibody

BrdU, Antibody
GWB-3AD5A4 0.1 mg Ask for price

BrdU Antibody
GWB-4088D4 0.02 mg Ask for price

BrdU Antibody
GWB-4A050B 0.1 mg Ask for price

BrdU Antibody
GWB-4B629C 0.5 ml Ask for price

BrdU Antibody
GWB-635992 0.25 mg Ask for price

BrdU Antibody
GWB-259463 0.05 mg Ask for price

BrdU Antibody
GWB-A46C9F 0.5 mg Ask for price

BrdU Antibody
GWB-4E7520 0.02 mg Ask for price

BrdU Antibody
GWB-E68456 0.2 mg Ask for price

BrdU Antibody
GWB-CA7B51 0.2 mg Ask for price

BrdU Antibody
GWB-D3BEFC 0.125 mg Ask for price

BrdU Antibody
GWB-D3FAF7 0.25 ml Ask for price

BrdU Antibody
GWB-D639BF 5 ml Ask for price

BRDU antibody
MBS831674-1mL 1mL
EUR 975

BRDU antibody
MBS831674-5x1mL 5x1mL
EUR 4235

BRDU antibody
MBS530403-01mg 0.1mg
EUR 655

BRDU antibody
MBS530403-5x01mg 5x0.1mg
EUR 2805

BRDU antibody
MBS534458-025mg 0.25mg
EUR 1180

BRDU antibody
MBS534458-5x025mg 5x0.25mg
EUR 5165

BRDU antibody
MBS534807-025mg 0.25mg
EUR 925

BRDU antibody
MBS534807-5x025mg 5x0.25mg
EUR 4010

BRDU (Bromodeoxyuridine)
PC-059 Polyclonal Ask for price

BrdU (Bromodeoxyuridine)
MC-328 IIB5 Ask for price

Bromodeoxyuridine (Brdu)
MM-1006-01 0.5 ml
EUR 158.9

Bromodeoxyuridine (Brdu)
MM-1006-02 1.0 ml
EUR 238.7

BrdU | Bromodeoxyuridine
AS16-3995 250 µg
EUR 407

Bromodeoxyuridine (BrdU)
MBS579325-1g 1g
EUR 175

Bromodeoxyuridine (BrdU)
MBS579325-200mg 200mg
EUR 140

Bromodeoxyuridine (BrdU)
MBS579325-500mg 500mg
EUR 150

Bromodeoxyuridine (BrdU)
MBS579325-50mg 50mg
EUR 135

Bromodeoxyuridine (BrdU)
MBS579325-5x1g 5x1g
EUR 635

Bromodeoxyuridine (BrdU)
MBS657820-10g 10g
EUR 610

Bromodeoxyuridine (BrdU)
MBS657820-1g 1g
EUR 190

Bromodeoxyuridine (BrdU)
MBS657820-25g 25g
EUR 925

Bromodeoxyuridine (BrdU)
MBS657820-5g 5g
EUR 395

Bromodeoxyuridine (BrdU)
MBS657820-5x25g 5x25g
EUR 4015

BrdU (bromodeoxyuridine)
MBS395162-01mg 0.1mg
EUR 425

BrdU (bromodeoxyuridine)
MBS395162-5x01mg 5x0.1mg
EUR 1760

BrdU (bromodeoxyuridine)
MBS395852-025mg 0.25mg
EUR 510

BrdU (bromodeoxyuridine)
MBS395852-5x025mg 5x0.25mg
EUR 2135

Bromodeoxyuridine (BrdU)
MBS396726-25mg 25mg
EUR 215

Bromodeoxyuridine (BrdU)
MBS396726-5x25mg 5x25mg
EUR 825

Bromodeoxyuridine (BrdU)
MBS438257-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

Bromodeoxyuridine (BrdU)
MBS438257-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

Bromodeoxyuridine (BrdU)
MBS438257-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

Bromodeoxyuridine (BrdU)
MBS438257-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

Bromodeoxyuridine (BrdU)
MBS438257-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

Bromodeoxyuridine (BrdU)
MBS3605189-100mg 100mg
EUR 200

Bromodeoxyuridine (BrdU)
MBS3605189-200mg 200mg
EUR 220

Bromodeoxyuridine (BrdU)
MBS3605189-5x200mg 5x200mg
EUR 680

BrdU IHC Kit
KT-077 50 slides
EUR 652

BrdU Mouse mAb
A1482 200μL
EUR 323.7
Description: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Complement factor B (CFB) (NP_001701.2).

BrdU Mouse mAb
A1482-100ul 100 ul
EUR 369.6

BrdU Mouse mAb
A1482-200ul 200 ul Ask for price

BrdU Mouse mAb
A1482-20ul 20 ul Ask for price

BrdU Mouse mAb
A1482-50ul 50 ul
EUR 267.6

BrdU Mouse mAb
E2264102 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

BrdU Mouse mAb
A21038 1000μL
EUR 828.95

BrdU Rabbit pAb
MBS9145457-005mL 0.05mL
EUR 235

BrdU Rabbit pAb
MBS9145457-5x005mL 5x0.05mL
EUR 885

MOUSE ANTI BrdU
MBS216162-002mg 0.02mg
EUR 220

MOUSE ANTI BrdU
MBS216162-5x002mg 5x0.02mg
EUR 805

MOUSE ANTI BrdU
MBS212833-02mg 0.2mg
EUR 515

MOUSE ANTI BrdU
MBS212833-5x02mg 5x0.2mg
EUR 2145

Mouse Anti BrdU
MBS225663-01mg 0.1mg
EUR 365

Mouse Anti BrdU
MBS225663-5x01mg 5x0.1mg
EUR 1465

BrdU Rabbit pAb
A20304 200μL
EUR 623.48

BrdU Rabbit mAb
A20790 500μL
EUR 1657.89

PE BrdU antibody
E16FXP069-100 100 tests
EUR 1191.67
Description: Available in various conjugation types.

Rabbit Anti BrdU
MBS225570-005mg 0.05mg
EUR 455

Rabbit Anti BrdU
MBS225570-5x005mg 5x0.05mg
EUR 1870

APC BrdU antibody
E16FXP070-100 100 tests
EUR 1235
Description: Available in various conjugation types.

BrdU Antibody (HRP)
GWB-Q01372 0.5 mg Ask for price

BrdU Antibody (HRP)
GWB-Q01373 0.1 mg Ask for price

BRDU antibody (FITC)
61R-1900 1 ml
EUR 221
Description: Mouse monoclonal BRDU antibody (FITC)

anti- BrdU antibody
FNab00952 100µg
EUR 658.5
Description: Antibody raised against BrdU

BrdU Antibody (FITC)
GWB-2B6986 0.25 ml Ask for price

BrdU Antibody (FITC)
GWB-149D93 0.05 mg Ask for price

BrdU Antibody (FITC)
GWB-A0B6BA 1 ml Ask for price

BrdU Antibody (FITC)
GWB-760419 0.5 ml Ask for price

BrdU (Bu20a) Antibody
48275-100ul 100ul
EUR 399.6

BrdU (Bu20a) Antibody
48275-50ul 50ul
EUR 286.8

BrdU (Bu20a) Antibody
MBS9435145-005mL 0.05mL
EUR 325

BrdU (Bu20a) Antibody
MBS9435145-01mL 0.1mL
EUR 445

BrdU (Bu20a) Antibody
MBS9435145-5x01mL 5x0.1mL
EUR 1860

Brdu 250 nm 2 ml
c18-250 2 ML
EUR 420

BrdU Mouse Monoclonal
MBS767184-01mg 0.1mg
EUR 330

BrdU Mouse Monoclonal
MBS767184-5x01mg 5x0.1mg
EUR 1315

BrdU Cell Proliferation
MBS396129-1000Tests 1000Tests
EUR 810

BrdU Cell Proliferation
MBS396129-5x1000Tests 5x1000Tests
EUR 3730

BrdU Cell Proliferation
MBS396131-200Tests 200Tests
EUR 415

BrdU Cell Proliferation
MBS396131-5x200Tests 5x200Tests
EUR 1900

BrdU Monoclonal Antibody
MBS126158-002mL 0.02mL
EUR 190

BrdU Monoclonal Antibody
MBS126158-005mL 0.05mL
EUR 225

BrdU Monoclonal Antibody
MBS126158-01mL 0.1mL
EUR 295

BrdU Monoclonal Antibody
MBS126158-02mL 0.2mL
EUR 440

BrdU Monoclonal Antibody
MBS126158-5x02mL 5x0.2mL
EUR 1815

Brdu Monoclonal Antibody
E-AB-70248-120uL 120uL
EUR 260
Description: Unconjugated

Brdu Monoclonal Antibody
E-AB-70248-200uL 200uL
EUR 410
Description: Unconjugated

Brdu Monoclonal Antibody
E-AB-70248-60uL 60uL
EUR 160
Description: Unconjugated

Brdu Monoclonal Antibody
E-AB-70248-each each Ask for price
Description: Unconjugated

Brdu Monoclonal Antibody
MBS2556266-006mL 0.06mL
EUR 190

Brdu Monoclonal Antibody
MBS2556266-012mL 0.12mL
EUR 265

Brdu Monoclonal Antibody
MBS2556266-02mL 0.2mL
EUR 415

Brdu Monoclonal Antibody
MBS2556266-5x02mL 5x0.2mL
EUR 1835

Brdu Monoclonal Antibody
RD70449A-120uL 120μL
EUR 360
Description: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 
_x000D_
_x000D_
_x000D_

Brdu Monoclonal Antibody
RD70449A-200uL 200μL
EUR 630
Description: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 
_x000D_
_x000D_
_x000D_

Brdu Monoclonal Antibody
RD70449A-60uL 60μL
EUR 204
Description: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 
_x000D_
_x000D_
_x000D_

Bromodeoxyuridine (BrdU) (AP)
MBS6124593-01mL 0.1(mL
EUR 935

Bromodeoxyuridine (BrdU) (AP)
MBS6124593-5x01mL 5x0.1mL
EUR 4050

Bromodeoxyuridine (BrdU) (PE)
MBS6125761-01mL 0.1(mL
EUR 935

Bromodeoxyuridine (BrdU) (PE)
MBS6125761-5x01mL 5x0.1mL
EUR 4050

Bromodeoxyuridine (BrdU) (AP)
MBS6187996-01mL 0.1(mL
EUR 950

Bromodeoxyuridine (BrdU) (AP)
MBS6187996-5x01mL 5x0.1mL
EUR 4120

Bromodeoxyuridine (BrdU) (PE)
MBS6188096-01mL 0.1(mL
EUR 950

Bromodeoxyuridine (BrdU) (PE)
MBS6188096-5x01mL 5x0.1mL
EUR 4120

Bromodeoxyuridine (BrdU) (PE)
MBS6251374-01mL 0.1(mL
EUR 995

Bromodeoxyuridine (BrdU) (PE)
MBS6251374-5x01mL 5x0.1mL
EUR 4325

Bromodeoxyuridine (BrdU) (AP)
MBS6247407-01mL 0.1(mL
EUR 995

Bromodeoxyuridine (BrdU) (AP)
MBS6247407-5x01mL 5x0.1mL
EUR 4325

Bromodeoxyuridine (BrdU) (AP)
MBS6257851-01mL 0.1mL
EUR 1090

Bromodeoxyuridine (BrdU) (AP)
MBS6257851-5x01mL 5x0.1mL
EUR 4765

Bromodeoxyuridine (BrdU)-PE
MBS6258893-01mL 0.1mL
EUR 1090

Bromodeoxyuridine (BrdU)-PE
MBS6258893-5x01mL 5x0.1mL
EUR 4765

Bromodeoxyuridine (BrdU) (APC)
MBS6124826-01mL 0.1(mL
EUR 935

Bromodeoxyuridine (BrdU) (APC)
MBS6124826-5x01mL 5x0.1mL
EUR 4050

Bromodeoxyuridine (BrdU) (HRP)
MBS6125528-01mL 0.1(mL
EUR 935

Bromodeoxyuridine (BrdU) (HRP)
MBS6125528-5x01mL 5x0.1mL
EUR 4050

Bromodeoxyuridine (BrdU) (APC)
MBS6188016-01mL 0.1(mL
EUR 950

Bromodeoxyuridine (BrdU) (APC)
MBS6188016-5x01mL 5x0.1mL
EUR 4120

Bromodeoxyuridine (BrdU) (HRP)
MBS6188076-01mL 0.1(mL
EUR 950

Bromodeoxyuridine (BrdU) (HRP)
MBS6188076-5x01mL 5x0.1mL
EUR 4120

Bromodeoxyuridine (BrdU) (HRP)
MBS6250347-01mL 0.1(mL
EUR 995

Bromodeoxyuridine (BrdU) (HRP)
MBS6250347-5x01mL 5x0.1mL
EUR 4325

A chemical modifier was mixed with the cIEF effluent in the flow-throw microvial to maintain the ESI stability and to mitigate ion suppression from the co-eluted carrier ampholytes and glycerol. Analysis of mAb samples have shown relative populations of two basic variants originating from C-terminal lysine process and acidic variant of deamidation.

The lysine clippings, deamidation, and sialic acid modification in oligosaccharide chains were revealed in infliximab. Two lysine clipping variants and a deamidated variant were observed in adalimumab. The duplicate analyses of a reference mAb demonstrated five charge variants separated by cIEF due to some unidentified modifications, as their mass spectra shared close similarities.

The mAb analyses demonstrated the feasibility of the cIEF-MS method, and they demonstrated how charge and structural variants and minor differences in therapeutic mAbs are observed with this technology. Online cIEF-MS is an information rich technology with high throughput, demonstrated by the initial data presented here.