Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time-of-flight mass spectrometry.

Capillary isoelectric focusing (cIEF) was online coupled to a Q-TOF MS by a flow-through microvial interface for the analysis of therapeutic mAb.

Intact molecular weights obtained from the mass spectrum deconvolution of separated charge variants provided information on the structural heterogeneity of therapeutic mAbs. A sandwich cIEF-MS configuration composed of anolyte, sample, and catholyte segments sequentially injected into a neutrally coated capillary was used for the charge heterogeneity separation of four mAbs.

Acetic acid and ammonium hydroxide were used in places of the non-volatile acids and bases commonly used for IEF but are incompatible with online MS detection. Glycerol was added as the anti-convective reagent.

5-BrdU
B3589-5.1 10 mM (in 1mL DMSO)
EUR 113
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

5-BrdU
B3589-500 500 mg
EUR 108
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

anti-BrdU
BROF-100T 100 test
EUR 537.5

BRDU antibody
20R-BS001 250 ug
EUR 553
Description: Sheep polyclonal BRDU antibody

BRDU antibody
10R-6763 1 ml
EUR 402
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-7945 100 ug
EUR 370
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-B121a 100 ug
EUR 445
Description: Mouse monoclonal BRDU antibody

BRDU antibody
20-BS17 250 ug
EUR 386
Description: Sheep polyclonal BRDU antibody

BRDU antibody
10R-2321 1 mL
EUR 601
Description: Mouse monoclonal BRDU antibody

BRDU antibody
10R-2356 1.5 mL
EUR 490
Description: Mouse monoclonal BRDU antibody

Sheep IS ELISA Kit
ESI0046 96Tests
EUR 521

Anserini IS ELISA Kit
EAI0046 96Tests
EUR 521

Rat IS ELISA Kit
ERI0046 96Tests
EUR 521

Rabbit IS ELISA Kit
ERTI0046 96Tests
EUR 521

Mouse IS ELISA Kit
EMI0046 96Tests
EUR 521

Monkey IS ELISA Kit
EMKI0046 96Tests
EUR 521

Porcine IS ELISA Kit
EPI0046 96Tests
EUR 521

Human IS ELISA Kit
EHI0046 96Tests
EUR 521

Canine IS ELISA Kit
ECI0046 96Tests
EUR 521

Chicken IS ELISA Kit
ECKI0046 96Tests
EUR 521

Bovine IS ELISA Kit
EBI0046 96Tests
EUR 521

Goat IS ELISA Kit
EGTI0046 96Tests
EUR 521

IS ELISA KIT|Human
EF007501 96 Tests
EUR 689

Anti-BrdU antibody
STJ22838 100 µl
EUR 277

Anti-BrdU antibody
STJ16101064 100 µg
EUR 354

Anti-BrdU Purified
11-286-C025 0.025 mg
EUR 108

Anti-BrdU Purified
11-286-C100 0.1 mg
EUR 177

Anti-BrdU Purified
11-682-C025 0.025 mg
EUR 108

Anti-BrdU Purified
11-682-C100 0.1 mg
EUR 177

Anti-BrdU PE
1P-682-T025 25 tests
EUR 140

Anti-BrdU PE
1P-682-T100 100 tests
EUR 240

Bromodeoxyuridine (BrdU) Antibody
abx000064-100ul 100 ul
EUR 411

BrdU Mouse mAb
A1482-100ul 100 ul
EUR 308

BrdU Mouse mAb
A1482-200ul 200 ul Ask for price

BrdU Mouse mAb
A1482-20ul 20 ul Ask for price

BrdU Mouse mAb
A1482-50ul 50 ul
EUR 223

Bromodeoxyuridine (BrdU) Antibody
abx412356-50ug 50 ug
EUR 509

Bromodeoxyuridine (BrdU) Antibody
abx414257-02mg 0.2 mg
EUR 565

Bromodeoxyuridine (BrdU) Antibody
abx414259-01mg 0.1 mg
EUR 439

Bromodeoxyuridine (BrdU) Antibody
abx414260-20ug 20 ug
EUR 272

Bromodeoxyuridine (BrdU) Antibody
abx230952-100ug 100 ug
EUR 509

anti- BrdU antibody
FNab00952 100µg
EUR 548.75
Description: Antibody raised against BrdU

BrdU (Bu20a) Antibody
48275-100ul 100ul
EUR 333

BrdU (Bu20a) Antibody
48275-50ul 50ul
EUR 239

Anti-BrdU Antibody
A1284-100
EUR 398

BRDU antibody (FITC)
61R-1900 1 ml
EUR 409
Description: Mouse monoclonal BRDU antibody (FITC)

Guinea Pig IS ELISA Kit
EGI0046 96Tests
EUR 521

Sheep BrDU ELISA Kit
ESB0693 96Tests
EUR 521

Mouse BrDU ELISA Kit
EMB0693 96Tests
EUR 521

Anserini BrDU ELISA Kit
EAB0693 96Tests
EUR 521

Rabbit BrDU ELISA Kit
ERTB0693 96Tests
EUR 521

Monkey BrDU ELISA Kit
EMKB0693 96Tests
EUR 521

Porcine BrDU ELISA Kit
EPB0693 96Tests
EUR 521

Rat BrDU ELISA Kit
ERB0693 96Tests
EUR 521

Human BrDU ELISA Kit
EHB0693 96Tests
EUR 521

Canine BrDU ELISA Kit
ECB0693 96Tests
EUR 521

Chicken BrDU ELISA Kit
ECKB0693 96Tests
EUR 521

Goat BrDU ELISA Kit
EGTB0693 96Tests
EUR 521

Bovine BrDU ELISA Kit
EBB0693 96Tests
EUR 521

BrdU (Bromodeoxyuridine)(BRD469) Antibody
BNUM0469-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), 1mg/mL

BrdU (Bromodeoxyuridine)(BRD494) Antibody
BNUM0494-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), 1mg/mL

BrdU (Bromodeoxyuridine)(BU20a) Antibody
BNUM0522-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), 1mg/mL

BrdU (Bromodeoxyuridine)(BRD469) Antibody
BNUB0469-100 100uL
EUR 209
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BRD469) Antibody
BNUB0469-500 500uL
EUR 458
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BRD494) Antibody
BNUB0494-100 100uL
EUR 209
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BRD494) Antibody
BNUB0494-500 500uL
EUR 458
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BU20a) Antibody
BNUB0522-100 100uL
EUR 209
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BU20a) Antibody
BNUB0522-500 500uL
EUR 458
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), Concentration: 0.2mg/mL

Bromodeoxyuridine (BrdU) Antibody (FITC)
abx414258-50ug 50 ug
EUR 342

Bromodeoxyuridine (BrdU) Antibody (FITC)
abx201100-100tests 100 tests
EUR 620

BrdU (Bu20a) Conjugated Antibody
C48275 100ul
EUR 397

Mouse BrdU ELISA Kit
ELA-E1336m 96 Tests
EUR 865

Human Serum Indoxyl Sulfate (IS) ELISA Kit
abx257961-96tests 96 tests
EUR 746

Human IS(serum indoxyl sulfate) ELISA Kit
EH4913 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml

Mouse Bromodeoxyuridine(BrdU)ELISA Kit 　
GA-E0163MS-48T 48T
EUR 336

Mouse Bromodeoxyuridine(BrdU)ELISA Kit 　
GA-E0163MS-96T 96T
EUR 534

Rat Bromodeoxyuridine(BrdU)ELISA Kit
GA-E0659RT-48T 48T
EUR 317

Rat Bromodeoxyuridine(BrdU)ELISA Kit
GA-E0659RT-96T 96T
EUR 496

Human Bromodeoxyuridine(BrdU)ELISA Kit
GA-E1920HM-48T 48T
EUR 289

Human Bromodeoxyuridine(BrdU)ELISA Kit
GA-E1920HM-96T 96T
EUR 466

Rat Anti Brdu Monoclonal Antibody
DMABT-50291RB 20 µg
EUR 429

Rat Anti Brdu Monoclonal Antibody
DMABT-50292RB 0.2 mg
EUR 741

Rat Anti Brdu Monoclonal Antibody
DMABT-50293RB 0.1 mg
EUR 663

Mouse Anti Brdu Monoclonal Antibody
DMABT-50295MB 20 µg
EUR 445

Mouse Anti Brdu Monoclonal Antibody
DMABT-50296MB 0.2 mg
EUR 715

Rat Anti Brdu Monoclonal Antibody
DMABT-50297RB 0.25 ml
EUR 1173

Rat Anti Brdu Monoclonal Antibody
DMABT-50299RB 0.25 mg
EUR 751

Rat Anti Brdu Monoclonal Antibody
DMABT-50300RB 0.5 mg
EUR 1193

Rat Anti Brdu Monoclonal Antibody
DMABT-50301RB 5 ml
EUR 892

Guinea Pig BrDU ELISA Kit
EGB0693 96Tests
EUR 521

BrdU Cell Proliferation Assay Kit
C0300-050 500 Assays
EUR 928

BrdU Cell Proliferation Assay Kit
C0300-100 1000 Assays
EUR 1312

BrdU Cell Proliferation Assay Kit
C0300-500 5000 Assays
EUR 4438

Human Bromodeoxyuridine,BrdU ELISA Kit
201-12-1904 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

5-Bromo-2'-deoxyuridine (BrdU)
40024 100MG
EUR 109
Description: Minimum order quantity: 1 unit of 100MG

CytoSelect BrdU Competitive ELISA Kit
CBA-5098 96 assays
EUR 543

A chemical modifier was mixed with the cIEF effluent in the flow-throw microvial to maintain the ESI stability and to mitigate ion suppression from the co-eluted carrier ampholytes and glycerol. Analysis of mAb samples have shown relative populations of two basic variants originating from C-terminal lysine process and acidic variant of deamidation.

The lysine clippings, deamidation, and sialic acid modification in oligosaccharide chains were revealed in infliximab. Two lysine clipping variants and a deamidated variant were observed in adalimumab. The duplicate analyses of a reference mAb demonstrated five charge variants separated by cIEF due to some unidentified modifications, as their mass spectra shared close similarities.

The mAb analyses demonstrated the feasibility of the cIEF-MS method, and they demonstrated how charge and structural variants and minor differences in therapeutic mAbs are observed with this technology. Online cIEF-MS is an information rich technology with high throughput, demonstrated by the initial data presented here.