Online Control of Cell Culture Redox Potential Prevents Antibody Interchain Disulfide Bond Reduction.

The phenomenon of monoclonal antibody (mAb) interchain disulfide bond reduction during manufacturing processes continues to be a focus of the biotechnology industry due to the potential for loss of product, increased complexity of purification processes, and reduced stability of the drug product.

We hypothesized that antibody reduction can be mitigated by controlling the cell culture redox potential and subsequently established a threshold redox potential above which the mAb remained intact and below which there were significant and highly variable amounts of reduced mAb. Using this knowledge, we developed three control schemes to prevent mAb reduction in the bioreactor by controlling the cell culture redox potential via an online redox probe.

Rat IS ELISA Kit

ERI0046 96Tests
EUR 521

Rabbit IS ELISA Kit

ERTI0046 96Tests
EUR 521

Anserini IS ELISA Kit

EAI0046 96Tests
EUR 521

Goat IS ELISA Kit

EGTI0046 96Tests
EUR 521

Canine IS ELISA Kit

ECI0046 96Tests
EUR 521

Chicken IS ELISA Kit

ECKI0046 96Tests
EUR 521

Human IS ELISA Kit

EHI0046 96Tests
EUR 521

Bovine IS ELISA Kit

EBI0046 96Tests
EUR 521

IS ELISA KIT|Human

EF007501 96 Tests
EUR 689

Guinea Pig IS ELISA Kit

EGI0046 96Tests
EUR 521

Human Serum Indoxyl Sulfate (IS) ELISA Kit

abx257961-96tests 96 tests
EUR 746

Human IS(serum indoxyl sulfate) ELISA Kit

EH4913 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml

In-situ adapter for use with T5000-96

T5000-A-IS 1 PC
EUR 2550.59

Rabbit Phosphorylase b kinase regulatory subunit alpha, liver is

ELI-08786Ra 96 Tests
EUR 928

Bone Glaprotein (BGP)

E62K004 20ug
EUR 343

Bone Matrix Proteins

5-00844 4 x 5mg Ask for price

Bone Matrix Proteins

20-abx265965
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg

Bone Sialoprotein Antibody

45125-100ul 100ul
EUR 252

Bone Sialoprotein Antibody

45125-50ul 50ul
EUR 187

Bone Sialoprotein Antibody

DF7738 200ul
EUR 304
Description: Bone Sialoprotein Antibody detects endogenous levels of total Bone Sialoprotein.

Bone Sialoprotein Antibody

ABD7738 100 ug
EUR 438

Bone Marrow antibody

10R-7946 100 ug
EUR 489
Description: Mouse monoclonal Bone Marrow antibody

Bone Sialoprotein (BSP) Antibody

20-abx171455
  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug

Bone Sialoprotein (IBSP) Antibody

20-abx216122
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Bone Sialoprotein (BSP) Antibody

20-abx175605
  • EUR 1149.00
  • EUR 565.00
  • 1 mg
  • 200 ug

Bone Sialoprotein (BSP) Antibody

20-abx175606
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Bone Sialoprotein (BSP) Antibody

20-abx175607
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Bone Sialoprotein Conjugated Antibody

C45125 100ul
EUR 397

Recombinant Bone Sialoprotein (BSP)

4-RPB092Hu01
  • EUR 504.99
  • EUR 238.00
  • EUR 1618.72
  • EUR 606.24
  • EUR 1112.48
  • EUR 401.00
  • EUR 3896.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Bone Sialoprotein expressed in: E.coli

Bone Sialoprotein Blocking Peptide

DF7738-BP 1mg
EUR 195

Bone Tissue Slide (Chondrosarcoma)

12-907-10um 10 um
EUR 201.5

Bone Tissue Slide (Chondrosarcoma)

12-907-4um 4 um
EUR 180.5

Bone Cytoplasmic Tumor Lysate

XBL-10492 0.1 mg
EUR 227.75
Description: Human bone tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human bone tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated bone tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated bone tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Bone Membrane Tumor Lysate

XBL-10493 0.1 mg
EUR 852.5
Description: Human bone tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human bone tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated bone tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated bone tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies

KLH11-G-5 5 ml
EUR 529

TruStrip RDT Clenbuterol rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100010-RDT 1 pk Ask for price

TruStrip RDT Quinolone rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100020-RDT 1 pk Ask for price

TruStrip RDT Salbutamal rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100030-RDT 1 pk Ask for price

TruStrip RDT Chloramphenicol rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100040-RDT 1 pk
EUR 651

TruStrip RDT Sulfanethazine rapid test card, results is 2-10 mins, 50 cards/pk

DE-100100-RDT 1 pk Ask for price

TruStrip RDT Ractopamine rapid test strips, results is 2-10 mins, 50 strips/pk

DE-10020-RDT 1 pk Ask for price

TruStrip RDT Gentamicin rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100200-RDT 1 pk Ask for price

TruStrip RDT Streptomycin rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100210-RDT 1 pk Ask for price

TruStrip RDT Melamine rapid test strips, results is 2-10 mins, 50 cards/pk

DE-100270-RDT 1 pk Ask for price

TruStrip RDT Lincomycin rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100630-RDT 1 pk Ask for price

TruStrip RDT Kanamycin rapid test strips, results is 2-10 mins, 50 strips/pk

DE-850101-RDT 1 pk Ask for price

Bone Dissociation System 1 (Chondrocytes, Neonatal bone, Osteoblastlike cells), Mouse and Rat

4-20211 ea Ask for price

Anti-Bone Sialoprotein/IBSP Antibody

PA1505 100ug/vial
EUR 334

Anti-Bone Sialoprotein/IBSP Antibody

PA1887 100ug/vial
EUR 334

Bone morphogenetic protein 2 (BMP2)

E62K008 20ug
EUR 343

Bone morphogenetic protein 7 (BMP7)

E62K009 20ug
EUR 343

Bone Sialoprotein 2 (IBSP) Antibody

20-abx126940
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Bone Marrow Proteoglycan (PRG2) Antibody

20-abx114889
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Mouse Bone PrimaCell: Normal Osteoblasts

2-82009 1 Kit Ask for price

Rat Bone PrimaCell: Normal Osteoblasts

2-82510 1 Kit Ask for price

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

Bone Marrow Proteoglycan (PRG2) Antibody

20-abx001972
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Bone Sialoprotein 2 (IBSP) Antibody

abx027806-400ul 400 ul
EUR 523

Bone Sialoprotein 2 (IBSP) Antibody

abx027806-80l 80 µl
EUR 286

Bone Marrow Proteoglycan (PRG2) Antibody

abx032601-400ul 400 ul
EUR 523

Bone Marrow Proteoglycan (PRG2) Antibody

abx032601-80l 80 µl
EUR 286

Bone Marrow Proteoglycan (PRG2) Antibody

abx022500-100ug 100 ug
EUR 578

Bone Marrow Proteoglycan (PRG2) Antibody

abx415167-01mg 0.1 mg
EUR 439

Bone Morphogenetic protein-7 Protein

20-abx260413
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

Bone Morphogenetic protein-4 Protein

20-abx261737
  • EUR 328.00
  • EUR 3599.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Bone Morphogenetic protein-5 Protein

20-abx261756
  • EUR 3766.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Bone Morphogenetic protein-7 Protein

20-abx261876
  • EUR 328.00
  • EUR 4490.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Bone Morphogenetic protein-2 Protein

20-abx262193
  • EUR 328.00
  • EUR 4365.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Bone Marrow Proteoglycan (PRG2) Antibody

20-abx313009
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Bone Marrow Proteoglycan (PRG2) Antibody

abx236770-100ug 100 ug
EUR 551

Bone Marrow Proteoglycan (PRG2) Antibody

abx236771-100ug 100 ug
EUR 551

Bone sialoprotein 2 (IBSP) Antibody

20-abx211839
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Bone sialoprotein 2 (IBSP) Antibody

20-abx212189
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Bone Marrow Proteoglycan (PRG2) Antibody

20-abx141483
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

Mouse Bone Sialoprotein (BSP) Protein

20-abx652699
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Bone Sialoprotein (BSP) Protein

20-abx652700
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Bone Sialoprotein (BSP) Protein

20-abx652149
  • EUR 704.00
  • EUR 286.00
  • EUR 2179.00
  • EUR 843.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Bone sialoprotein 2 (IBSP)

1-CSB-EP010945HU(A4)
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Bone sialoprotein 2 (IBSP),partial expressed in E.coli

Human Bone marrow proteoglycan (PRG2)

1-CSB-CF018670HU
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human Bone marrow proteoglycan(PRG2),partial expressed in in vitro E.coli expression system

Human Bone marrow proteoglycan (PRG2)

1-CSB-CF018670HUa2
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human Bone marrow proteoglycan(PRG2),partial expressed in in vitro E.coli expression system

Immortalized Human Bone Marrow Basophils

T0527 1x106 cells / 1.0 ml Ask for price

Dog Bone Sialoprotein ELISA kit

E08B0392-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Bone Sialoprotein ELISA kit

E08B0392-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Bone Sialoprotein ELISA kit

E08B0392-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bone Sialoprotein ELISA kit

E09B0392-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bone Sialoprotein ELISA kit

E09B0392-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bone Sialoprotein ELISA kit

E09B0392-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Bone Sialoprotein ELISA kit

E04B0392-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Bone Sialoprotein ELISA kit

E04B0392-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Bone Sialoprotein ELISA kit

E04B0392-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Bone Sialoprotein ELISA kit

E03B0392-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Bone Sialoprotein ELISA kit

E03B0392-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Bone Sialoprotein ELISA kit

E03B0392-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bone Sialoprotein ELISA kit

E07B0392-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bone Sialoprotein ELISA kit

E07B0392-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bone Sialoprotein ELISA kit

E07B0392-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Bone Sialoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

These control methodologies functioned by increasing the concentration of dissolved oxygen (DO), copper (II) (Cu), or both DO and Cu to maintain the redox potential above the threshold value. Using these methods, we were able to demonstrate successful control of antibody reduction. Importantly, the redox control strategies did not significantly impact the cell growth, viability, mAb production, or product quality attributes including aggregates, C-terminal lysine, high mannose, deamidation and glycation.

Our results demonstrate that controlling the cell culture redox potential is a simple and effective method to prevent mAb reduction. This article is protected by copyright. All rights reserved.